Posttranslational Modifications of Proteins
| Автор(ы): | Kannicht C.
06.10.2007
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| Год изд.: | 2002 |
| Описание: | Posttranslational Modifications of Proteins: Tools for Functional Proteomics is a compilation of detailed protocols needed to detect and analyze the most important co- and posttranslational modifications of proteins. Though, for reasons of simplicity not explicitly mentioned in the title, both kinds of modifications are covered, whether they occur during, or after, biosynthesis of the protein. My intention was to coverthe most significant protein modifications, focusing on the fields of protein function, proteome research, and the characterization of pharmaceutical proteins. The majority of all proteins undergo co- and/or posttranslational modifications. Knowledge of these modifications is extremely important, since they may alter physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, function ofthe proteins. Moreover, the modification itself can act as an added functional group. Full understanding of a specific protein structure-function relationship requires detailed information not only on its amino acid sequence, which is determined by the corresponding DNA sequence, but also on the presence and structure of protein modifications. |
| Оглавление: |
Обложка книги.
Jiang Wu and J. ThrockWatson [1] 2 Carbohydrate Composition Analysis of Glycoproteins Using Highly Sensitive Fluorescence Detection Methods George N. Saddle, Mary Beth Ebert, Shirish T. Dhume, and Kalyan R. Anumula [23] 3 Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB) Rolf Nuck [37] 4 Separation of N-Glycans by HPLC Martin Gohlke [45] 5 Enzymatic Sequence Analysis of N-Glycans Christoph Kannicht and Anke Flechner [63] 6 Immunological Detection of O-GlcNAc Monika Rex-Mathes, Jurgen Koch, Sabine Werner, Lee S. Griffith, and Brigitte Schmitz [73] 7 Analysis of O-Glycosylation Juan J. Calvete and Libia Sanz [89] 8 Characterization of Site-Specific Glycosylation Katalin F. Medzihradszky [101] 9 Monitoring Glycosylation of Therapeutic Glycoproteins for Consistency Using Highly Fluorescent Anthranilic Acid Shirish T. Dhume, Mary Beth Ebert, George N. Saddle, and Kalyan R. Anumula [127] 10 Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa Peter Gerold and Ralph T. Schwarz [143] 11 Analysis of S-Acylation of Proteins Michael Veit, Evgeni Ponimaskin, and Michael F. G. Schmidt [159] 12 Immunoblotting Methods for the Study of Protein Ubiquitination Edward G. Mimnaugh and Leonard M. Neckers [179] 13 Analysis of Methylation and Acetylation in E. coll Ribosomal Proteins Randy J. Arnold and James P. Reilly [205] 14 Identification of In Vivo Protein Phosphorylation Sites with Mass Spectrometry Jun Qin and Xiaolong Zhang [211] 15 Analysis of Tyrosine-O-Sulfation Jens R. Bundgaard, Anders H. Johnsen, and Jens F. Rehfeld [223] 16 (?)-Amidated Peptides: Approaches for Analysis Gregory P. Mueller and William J. Driscoll [241] 17 (?)-Glutamate and (?)-Hydroxyaspartate in Proteins Francis J. Castellino, Victoria A. Ploplis, and Li Zhang [259] 18 Detection of isoAspartate Residues as a Posttranslational Modification of Proteins and Peptides Verne Schirch, Sonia Delle Fratte, and Marti no di Salvo [269] 19 Lysine Hydroxylation and Crosslinking of Collagen Mitsuo Yamauchi and Masashi Shiiba [277] 20 Heterologous Expression in Endocrine Cells for Analysis of Posttranslational Modifications Jens R. Bundgaard [291] 21 2D-Electrophoresis: Detection of Glycosylation and Influence on Spot Pattern Klemens Loster and Christoph Kannicht [301] Index [317] |
| Формат: | djvu |
| Размер: | 3221426 байт |
| Язык: | ENG |
| Рейтинг: |
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| Открыть: | Ссылка (RU) |