Apoptosis and Cell Proliferation

Автор(ы):Mannheim B.
06.10.2007
Год изд.:1998
Издание:2
Описание: This second edition of the guide reflects that progress in apoptosis research. It contains more information on apoptosis and describes more Roche Molecular Biochemicals products to make apoptosis research easier and faster. Some of the highlights of this edition are: An apoptosis pathways chart, which summarizes information from many laboratories, and a brief literature guide for those interested in learning more about apoptosis research; Method selection guides at the beginning of the apoptosis section and the cell proliferation chapter, to help you quickly find the Roche Molecular Biochemicals product that best fits your research needs; A separate section, within the cell death chapter, which spotlights those kits that can be used to measure cytotoxicity, regardless of whether the measured cell death is due to apoptosis or necrosis; More information on the use of flow cytometry to answer questions about cell death and cell proliferation. The authors added new information, however, they always kept the original purpose of the guide in mind. As with the first edition, this second edition is still designed to answer one question: What is the best way for you to get the answers you need in your apoptosis or cell proliferation research?
Оглавление:
Apoptosis and Cell Proliferation — обложка книги.
Chapter [1]
Cell Death - Apoptosis and Necrosis
  1.1 Introduction [2]
    1.1.1 Terminology of cell death [2]
    1.1.2 Differences between necrosis and apoptosis [3]
    1.1.3 Apoptotic Pathways [4]
  1.2 Apoptosis Assay Methods [6]
        Method/Product selection guide [7]
    1.2.1 Methods for studying apoptosis in cell populations [8]
      1.2.1.1 Assays that measure DMA fragmentation [8]
        Apoptotic DMA Ladder Kit [11]
        Cell Death Detection ELISAPLUS [13]
      1.2.1.2 Assays that measure apoptosis-induced proteases (caspases) [16]
        Caspase 3 Activity Assay [17]
        Anti-PARP [20]
      1.2.1.3 Summary of methods for studying apoptosis in cell populations [22]
    1.2.2 Methods for studying apoptosis in individual cells [24]
      1.2.2.1 The TUNEL enzymatic labeling assay [24]
        In SituCett Death Detection Kit, Fluorescein [27]
        In Situ Cell Death Detection Kit, AP [29]
        In Situ Cell Death Detection Kit, POD [29]
      1.2.2.2 Assays that measure membrane alterations [31]
        Annexin-V-FLOUS [32]
        Annexin-V-FLOUS Staining Kit [32]
        Annexin-V-Alexa™ 568 [32]
        Annexin-V-Biotin [34]
      1.2.2.3 Assays that use DMA stains [36]
        DAPI, Ethidium bromide, Propidium iodide [36]
      1.2.2.4 Summary of methods for studying apoptosis in individual cells [38]
    1.2.3 Detection of apoptosis-related proteins [40]
        Anti-Fas (CD95/Apo-1) [41]
        Anti-Fas-Biotin (CD95/Apo-1) [42]
        Anti-Bcl-2 oncoprotein, human [44]
        Anti-p53-Protein, mutant [46]
        Anti-p53-Protein pan [46]
        Anti-p53 pan [46]
        Anti-p53, Biotin labeled [46]
        Anti-p53, Peroxidase labeled [46]
        p53 pan ELISA [48]
  1.3 Cytotoxicity Assay Methods [50]
    1.3.1 Relationship between cytotoxicity, apoptosis and necrosis [50]
    1.3.2 Methods for studying cytotoxicity [50]
      1.3.2.1 Assays that measure plasma membrane leakage [51]
        Cytotoxicity Detection Kit (LDH) [52]
        Cellular DMA Fragmentation ELISA [54]
      1.3.2.2 Assays that measure metabolic activity [58]
        Cell Proliferation Kit I (MTT) [58]
        Cell Proliferation Kit II (XTT) [58]
        Cell Proliferation Reagent WST-1 [58]
      1.3.2.3 Summary of methods for studying cytotoxicity [60]
Chapter 2
Cell Proliferation and Viability
  2.1 Introduction [64]
    2.1.1 Terminology of cell proliferation and viability [64]
    2.1.2 Cell cycle [64]
  2.2 Cell proliferation/viability assay methods _ Method/Product selection guide [68]
    2.2.1 Methods for studying cell proliferation and viability in cell populations [70]
      2.2.1.1 Assays that measure metabolic activity [70]
        Cell Proliferation Kit I (МП) [73]
        Cell Proliferation Kit II (ХП) [74]
        Cell Proliferation Reagent WST-1 [75]
      2.2.1.2 Assays that measure DMA synthesis [77]
        BrdU Labeling and Detection Kit III [79]
        Cell Proliferation ELISA, BrdU (colorimetric) [81]
        Cell Proliferation ELISA, BrdU (chemiluminescence) [81]
      2.2.1.3 Summary of methods for studying cell proliferation and cell viability in cell populations [84]
      2.2.1.4 Single reagents for the measurement of DMA synthesis [84]
    2.2.2 Methods for studying cell proliferation and viability in individual cells [86]
      2.2.2.1 Assays that measure DMA synthesis [86]
        BrdU Labeling and Detection Kit I [87]
        BrdU Labeling and Detection Kit II [87]
        In SituCett Proliferation Kit, FLUOS [88]
        In Situ Cell Proliferation Kit, AP [90]
        Anti-BrdU, formalin grade [93]
        Anti-BrdU-Fluorescein [93]
        Anti-BrdU-AP, F(ab')2 fragments [93]
        Anti-BrdU-Peroxidase, Fab fragment [93]
      2.2.2.2 Assays that monitor expression of cell cycle-associated antigens [96]
        Monoclonal antibodies to cell cycle-associated antigens [97]
      2.2.2.3 Summary of methods for studying cell proliferation and viability in individual cells [100]
Chapter 3
Appendix
  3.1 Technical tips [104]
    3.1.1 Selected frequently asked questions (FAQs) about cell death assays [104]
    3.1.2 Technical tips on the TUNEL method [105]
      3.1.2.1 TUNEL: Improvement and evaluation of the method for in situ; apoptotic cell identification [105]
      3.1.2.2 TUNEL protocol for tissues which tend to give false positives [105]
      3.1.2.3 Tips for avoiding or eliminating potential TUNEL labeling artifacts [107]
    3.1.3 Technical tips on the use of Annexin-V-Biotin for light microscope detection [109]
    3.1.4 Technical tips on the use of the Apoptotic DNA Ladder Kit on tissue samples [109]
    3.1.5 Technical tips on the Cell Proliferation ELISA kits [110]
  3.2 Special applications of cell death and cell proliferation methods [111]
    3.2.1 TUNEL assays [111]
      3.2.1.1 Discrimination between dead and viable apoptotic cells using two-color TdT assay and surface labeling as detected by flow cytometry [111]
      3.2.1.2 The use of flow cytometry for concomitant detection of apoptosis and cell cycle analysis [111]
      3.2.1.3 Comparison of two cell death detection methods: In situ nick translation and TUNEL [112]
      3.2.1.4 Fixation of tissue sections for TUNEL combined with staining for thymic epithelial cell marker [112]
    3.2.2 Metabolic assays [113]
      3.2.2.1 Biochemical and cellular basis of cell proliferation assays that use tetrazolium salts [113]
    3.2.3 Annexin assays [113]
      3.2.3.1 The use of annexin for concomitant detection of apoptosis and cellular phenotype [113]
    3.2.4 BrdU assays [114]
      3.2.4.1 Detection of bromodeoxyuridine in paraffin-embedded tissue sections using microwave antigen retrieval is dependent on the mode of tissue fixation [114]
    3.3.1 Apoptosis-related parameters - Abbreviations and References [115]
    3.3.2 Examples for applications of Boehringer Mannheim products [120]
    3.3.3 General references [127]
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